Physiological characterization of strain DCB-1, a unique dehalogenating sulfidogenic bacterium

The pentachlorophenol-dehalogenating Desulfitobacterium hafniense strain PCP-1.

In this report, a complete description of Desulfitobacterium hafniense strain PCP-1 is presented. The D. hafniense strain PCP-1 was isolated from a methanogenic consortium for its capacity to dehalogenate pentachlorophenol (PCP) into 3-chlorophenol. This strain is also capable of dehalogenating several other chloroaromatic compounds and tetrachloroethene into trichloroethene. Four gene loci enc...

Purification and characterization of 2,4,6-trichlorophenol-4-monooxygenase, a dehalogenating enzyme from Azotobacter sp. strain GP1.

The enzyme which catalyzes the dehalogenation of 2,4,6-trichlorophenol (TCP) was purified to apparent homogeneity from an extract of TCP-induced cells of Azotobacter sp. strain GP1. The initial step of TCP degradation in this bacterium is inducible by TCP; no activity was found in succinate-grown cells or in phenol-induced cells. NADH, flavin adenine dinucleotide, and O2 are required as cofacto...

Detection and characterization of a dehalogenating microorganism by terminal restriction fragment length polymorphism fingerprinting of 16S rRNA in a sulfidogenic, 2-bromophenol-utilizing enrichment.

Terminal restriction fragment length polymorphism analysis of reverse-transcribed 16S rRNA during periods of community flux was used as a tool to delineate the roles of the members of a 2-bromophenol-degrading, sulfate-reducing consortium. Starved, washed cultures were amended with 2-bromophenol plus sulfate, 2-bromophenol plus hydrogen, phenol plus sulfate, or phenol with no electron acceptor ...

Involvement of a Collar Structure in Polar Growth and Cell Division of Strain DCB-1.

Microscopic methods were used to investigate the unique collar structure of the gram-negative sulfate-reducing bacterium, strain DCB-1. Polar cell growth apparently occurred from the collar. When the daughter cell was approximately equal in length to the mother cell and the collar was thus centrally located, cell division occurred within the collar region. Division was by a novel mechanism whic...

Construction and Characterization of a Lux-Marked Phenanthrene Degrading Bacterium